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KMID : 1234420090370020125
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 2 p.125 ~ p.132
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Characterization of Antimicrobial Substance Produced by Lactobacillus sp. HN 235 Isolated from Pig Intestine
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Shin Myeong-Su
Han Sun-Kyung
Choi Ji-Hyun
Ji Ae-Ran
Kim Kyeong-Su
Lee Wan-Kyu
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Abstract
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In order to develop probiotics which may be a viable alternative of antibiotic use in pig industry, five bacterial strains (Lactobacillus sp. HN 52, 92, 98, 235 and AP 116) possessing antimicrobial properties were selected from 500 strains isolates of pig intestines. The bacteriocin produced by Lactobacillus sp. HN 235 displayed a relative broad spectrum of inhibitory activity against all Enterococcus strains, Pseudomonas aeruginosa, Listeria monocytogenes and Clostridium perfringens using the spot-on-lawn method. The production of antimicrobial substance started in the middle of the exponential growth phase, reached maximum levels (6,400 AU/ mL) in the stationary phase, and then declined. Bacteriocin activity remained unchanged after 30 min of heat treatment at 95oC and stable from pH 2.0 to 10 for 1 h, or exposure to organic solvents; however, it diminished after treatment with proteolytic enzymes. The molecular weight of the bacteriocin was about 5 kDa according to a tricine SDS-PAGE analysis.
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KEYWORD
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Antimicrobial substance, bacteriocin, lactic acid bacteria, probiotics, pig
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